MTOR 抗体
Applications
| Tested Applications: |
ELISA, WB, IHC, IP, IF |
| Cited applications |
IHC, WB |
| Specificity Reaction: |
Human,Mouse,Rat; Other species are not tested.Please decide the specificity by homology |
| Cited species |
Human |
| Positive WB detected in |
HeLa cells, HeLa/RPE1 cells, K-562 cells, MCF7 cells |
| Positive IP detected in |
HeLa cells |
| Positive IHC detected in |
Human testis tissue, human prostate cancer tissue |
| Positive IF detected in |
HeLa cells |
| Recommended dilution |
WB : 1:200-1:2000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200 |
MTOR 抗体Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
Images
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WB result of anti-mTOR (20657-1-AP) in different cell lysates.
HeLa cells were subjected to SDS PAGE followed by western blot with 20657-1-AP(MTOR antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using 20657-1-AP(MTOR antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using 20657-1-AP(MTOR antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-MTOR (IP:20657-1-AP, 5ug; Detection:20657-1-AP 1:500) with HeLa cells lysate 1200ug.
Immunofluorescent analysis of HeLa cells using 20657-1-AP( MTOR Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Product Information
| Source: |
Rabbit |
Purify: |
Antigen affinity purification |
| IsoType: |
IgG |
Storage: |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. -20oC, Avoid freeze / thaw cycles. |
Immunogen Information MTOR 抗体
| Immunogen: |
Peptide |
Full Name: |
FK506 binding protein 12-rapamycin associated protein 1 |
Caculated Molecular
Weight: |
289 kDa |
Observed Molecular
Weight: |
289 kDa |
| GenbankNo |
NM_004958 |
GeneID: |
2475 |
| Symbol |
FRAP1 |
Synonyms |
FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1 |
Background
|
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. The antibody is specific to MTOR. MTOR 抗体
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