人胆囊收缩素-试剂盒-抗体-生物在线
翌圣生物科技(上海)股份有限公司
人胆囊收缩素

人胆囊收缩素

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产品名称: 人胆囊收缩素

英文名称: Human Cholecystokinin ELISA Kit

产品编号: E0802h

产品价格: null

产品产地: USCNLIFE 原装

品牌商标: USCNLIFE

更新时间: 2024-12-10T13:16:28

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产品名称:人胆囊收缩素(cck)
英文名称:Human Cholecystokinin ELISA Kit
产品分类:人细胞因子ELISA试剂盒[Human cytokin ELISA Kit]
产品编号:E0802h
检验方法:ELISA
包 装:96T
价 格:3680
品 牌:USCNLIFE 原装
说明书下载:中文下载Instruction
产品说明:
 
预期应用
    ELISA法定量测定人血清、血浆或其它相关液体中CCK含量。
 
实验原理
    本试剂盒应用双抗体夹心酶标免疫分析法测定标本中CCK水平。用纯化的抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入CCK抗原、生物素化的抗人CCK抗体、HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CCK呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样品浓度。
 
Intended use
    This immunoassay kit allows for the specific measurement of human CCK concentrations in serum, and plasma.
 
Introduction
    Cholecystokinin(CCK) plays a key role in facilitating digestion within the small intestine. It is secreted from mucosal epithelial cells in the first segment of the small intestine (duodenum), and stimulates delivery into the small intestine of digestive enzymes from the pancreas and bile from the gallbladder. Cholecystokinin is also produced by neurons in the enteric nervous system, and is widely and abundantly distributed in the brain. Cholecystokinin and gastrin are highly similar peptides. Like gastrin, cholecystokinin is a linear peptide that is synthesized as a preprohormone, then proteolytically cleaved to generate a family of peptides having the same carboxy ends. Full biologic activity is retained in CCK-8 (8 amino acids), but peptides of 33, 38 and 59 amino acids are also produced. In all of these CCK peptides, the tyrosine seven residues from the end is sulfated, which is necessary for activity.