亨廷顿相互作用蛋白12(HIP12)重组蛋白

YB525Hu014
Recombinant Huntingtin Interacting Protein 12 (HIP12)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 亨廷顿相互作用蛋白12(HIP12)重组蛋白 PROPERTIES ]
Residues: Ser771~Ala1012
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: O75146
Host: E. coli
Subcellular Location: Cytoplasm, perinuclear
region. Endomembrane system. Cytoplasmic
vesicle, clathrin-coated vesicle membrane.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
 亨廷顿相互作用蛋白12(HIP12)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.3
Predicted Molecular Mass: 30.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
 亨廷顿相互作用蛋白12(HIP12)重组蛋白precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
SLDVRQEELG AVVDKEMAAT SAAIEDAVRR IEDMMNQARH ASSGVKLEVN ERILNSCTDL
MKAIRLLVTT STSLQKEIVE SGRGAATQQE FYAKNSRWTE GLISASKAVG WGATQLVEAA
DKVVLHTGKY EELIVCSHEI AASTAQLVAA SKVKANKHSP HLSRLQECSR TVNERAANVV
ASTKSGQEQI EDRDTMDFSG LSLIKLKKQE METQVRVLEL EKTLEAERMR LGELRKQHYV
LA