FITC标记的泛素连接酶蛋白CHFR抗体
产品名称： FITC标记的泛素连接酶蛋白CHFR抗体
英文名称： Anti-CHFR/FITC
产品编号： HZ-4272R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-CHFR/FITC Conjugated antibody
FITC标记的泛素连接酶蛋白CHFR抗体
| 英文名称 | Anti-CHFR/FITC |
| 中文名称 | FITC标记的泛素连接酶蛋白CHFR抗体 |
| 别 名 | Checkpoint with forkhead and ring finger domains; Checkpoint with forkhead and RING finger domains protein; E3 ubiquitin protein ligase CHFR; EC 6.3.2.; RING finger protein 196; RNF 116; RNF 196; RNF116; RNF196; Ubiquitin ligase protein; CHFR_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 染色质和核信号 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Rabbit, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 73 kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CHFR |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: A number of human cancers are sensitive to mitotic stress, implying checkpoint defects. Many proteins that contain forkhead-associated (FHA) domains are cell cycle checkpoints and CHFR is one such protein. It has a FHA and ring finger domain within its N terminus. Within its C terminus, CHFR contains a cysteine-rich region that did not display significant similarity to any protein in the GenBank database, but is highly conserved between humans and mice. It has been concluded that CHFR defines a checkpoint that delays entry into metaphase in response to mitotic stress. It has been found CpG methylation-dependent silencing of CHFR expression occurs in 45% of cancer cell lines, 40% of primary colorectal cancers, 53% of colorectal adenomas, and 30% of primary head and neck cancers. Function: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. Subunit: Interacts with HDAC1 and HDAC2. Interacts with PML (with sumoylated form of PML). Subcellular Location: Nucleus, PML body. Tissue Specificity: Ubiquitous. Post-translational modifications: Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1. Autoubiquitinated; may regulate its cellular level. Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity. Similarity: Belongs to the CHFR family. Contains 1 FHA domain. Contains 1 PBZ-type zinc finger. Contains 1 RING-type zinc finger. Database links: Entrez Gene: 55743 Human Entrez Gene: 231600 Mouse Omim: 605209 Human SwissProt: Q96EP1 Human SwissProt: Q810L3 Mouse Unigene: 720197 Human Unigene: 30264 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
许多人类癌症对有丝分裂应激敏感，这意味着检查点缺陷。许多含有叉头相关（FHA）结构域的蛋白质是细胞周期检查点，CHFR就是这样一种蛋白质。它有一个FHA和环指域在其N端。在其C端，CHFR含有富含半胱氨酸的区域，其与GenBank数据库中的任何蛋白质没有显示显著的相似性，但在人和小鼠之间高度保守。已经得出结论，CHFR定义一个检查点，延迟进入中期响应于有丝分裂应力。已发现CpG甲基化依赖性沉默CHFR表达发生在45%的癌细胞系，40%的原发性结直肠癌，53%的结直肠腺瘤，和30%的原发性头颈部癌症。