MLK3 抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
MLK3 抗体

MLK3 抗体

商家询价

产品名称： MLK3 抗体

英文名称： MLK3 Antibody

产品编号： XY11996-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 MLK3 抗体

Applications
In-house applications ELISA, WB, IHC, IF     Application not tested? Please see our Guarantee.
Species specificity Human,Mouse,Rat; other species not tested.
Images
Prev1NextView more validation data
  • 11996-1-AP;PC-3 cellPC-3 cells were subjected to SDS PAGE followed by western blot with 11996-1-AP(MAP3K11 antibody) at dilution of 1:200
  • 11996-1-AP;Colon cancerImmunohistochemical of paraffin-embedded human colon cancer using 11996-1-AP(MAP3K11 antibody) at dilution of 1:50 (under 10x lens)
  • 11996-1-AP;HelaImmunofluorescent analysis of Hela cells, using MAP3K11 antibody 11996-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Positive WB detected in PC-3 cells, HEK-293, Jurkat cells
Positive IHC detected in Human colon cancer tissue
Positive IF detected in Hela cells
Recommended dilution WB : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200
 MLK3 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information MLK3 抗体
Immunogen MLK3 fusion protein ag2636 Full name Mitogen-activated protein kinase kinase kinase 11
Calculated molecular
weight		 847aa,93 kDa Observed molecular 
weight		 60 kDa
GenBank accession 
number		 BC011263 Gene ID (NCBI) 4296
Gene symbol MAP3K11 Synonyms MAP3K11, Mixed lineage kinase 3, MLK 3, MLK3, PTK1, SPRK
Background

MLK3(Mixed lineage kinase 3) is also named as MAP3K11 and activates the JUN N-terminal pathway. It influences microtubule organization during the cell cycle. Mouse epididymal sperm contained an 60 kDa variant of MLK3, which may have been a result of proteolytic cleavage of the longer 93 kDa form seen in many tissues and the 50 kDa band is not known and not infrequently observed(PMID:17377140). Exposure of cells to LPS resulted in an increase in MLK3 dimers(of approximately 220 kDa), whereas inactivation of GSK-3b by TWS119 blocked MLK3 dimerization(PMID:21194439). MLK3 抗体

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